ABSTRACT
We have carried out the chemical investigation on the roots of Alangium chinense. The chemical constituents from the roots of A.chinense were isolated and purified by various chromatographic techniques, such as silica gel, MCI-Gel resin, Sephadex LH-20 and high performance liquid chromatography. As a result, three alkaloids (1-3) were isolated from 90% EtOH extracts of the roots of this plant. Their structures were elucidated by physical-chemical properties and spectral data. Among them, compound 1 is a new compound, determined as 8-hydroxy-3-hydroxymethyl-6,9-dimethyl-7H-benzo[de]isoquinolin-7-one. Cytotoxicity of the compounds was evaluated by the MTT method. Compound 1 displayed cytotoxicity against NB4, A-549, SHSY5Y, PC-3 and MCF-7 cell lines with IC₅₀ values of 4.2, 3.5, 5.7, 2.8 and 3.9 μmol•L⁻¹, respectively.
ABSTRACT
Objective: To study the chemical constituents in the twigs of Cassia fistula. Methods: The chemical constituents in the twigs of C. fistula were isolated by silica gel, Sephadex LH-20 column chromatography, and RP-HPLC methods. And their structures were elucidated by using various spectroscopic techniques, including extensive 1D and 2D NMR techniques. Results: A new tricyclic alkaloid, named as cassiarin L (1) was isolated from 95% ethanol extract of the twigs of C. fistula. Conclusion: Compound 1 is a new compound and it displays obvious cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 1.8, 2.6, 3.5, 4.2, and 2.3 μmol/L, respectively.
ABSTRACT
A new isoindole alkaloid (1), has been isolated from the leaves of Cassia siamea by using various chromatographic techniques. Compound 1 is a new compound, determined as 5-(hydroxymethyl)-2-methyl-6-prenylisoindolin-1-one, and it displayed cytotoxicity against NB4, A549, SHSY5Y, PC3 and MCF7 cell lines with IC₅₀ values of 3.2,4.6,2.8,6.4, 2.5 μmol•L⁻¹, respectively.
ABSTRACT
A new isocoumarin, along with five known ones,were isolated from the fermentation products of an endophytic fungus Aspergillus versicolorby using various chromatographic techniques.Their structures were elucidated on the basis of extensivespectroscopic analysis, including 1D-and 2D-NMR techniques. Compound 1 was evaluated for cytotoxicity against five human tumor cell lines. The results showed that 1 exhibited weak cytotoxicityagainst NB4, SHSY5Y and MCF7 cells with IC₅₀ values of 6.8, 4.3,8.8 μmol•L⁻¹, respectively.
ABSTRACT
For the purpose of finding new bioactive agents from ethnic medicines, the chemical study on Dai Medicine Cassia occidentalis was carried out. The chemical constituents from the seeds of C. occidentalis were isolated by column chromatographic methods on silica gel, MCI-Gel resin, Sephadex LH-20, and high performance liquid chromatography. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. The cytotoxicity of the compound for NB4, A549, SHSY5Y, PC3, and MCF7 cells line was also assayed by using the MTT method. Two sesquiterpenes (1 and 2) were isolated from this plant. Compound 1 is a new compound and named as methyl 6-(hydroxymethyl)-4-isopropyl-7-methoxynaphthalene-1-carboxylate. Compound 1 also displayed high cytotoxicity with the tested cancer cell-lines.
ABSTRACT
A new furan-2-carboxylic acid, 5-[3-(hydroxymethyl)-4,5-dimethoxyphenyl]-3-methylfuran-2-carboxylic acid(1),has been isolated from the bark of Cassia alata by using various chromatographic techniques. It displayed cytotoxicity against NB4, A549, SHSY5Y, PC3 and MCF7 cell lines with IC₅₀ values of 2.5, 1.2, 2.2, 3.6 and 1.9 μmol•L⁻¹, respectively.
ABSTRACT
Objective: To study the chemical constituents in the twigs of Cassia siamea. Methods: The chemical constituents in the twigs of C. siamea were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatographies and RP-HPLC methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results: A new anthraquinone, named as cassanthraquinone A (1) was isolated from 95% ethanol extract of the twigs of C. siamea. Conclusion: Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 6.2, 7.8, 3.5, 8.6, and 5.4 μmol/mL, respectively.
ABSTRACT
A new phenylpropanoid (1), together with seven known ones (2-8), has been isolated from the flowers of Rosa rugosa collected from Shanxi province by using various chromatographic techniques. Compound 1 is a new compound, and it displayed cytotoxicity against NB4, SH-SY5Y, PC3, A549 and MCF7 cell lines with IC₅₀ values of 8.2, 6.2, 4.3, 2.8, and 9.6 µmol · L⁻¹ respectively.
Subject(s)
Humans , Cell Line , Cell Survival , Drugs, Chinese Herbal , Chemistry , Pharmacology , Flowers , Chemistry , Molecular Structure , Phenylethyl Alcohol , Chemistry , Pharmacology , Rosa , Chemistry , Spectrometry, Mass, Electrospray IonizationABSTRACT
A phytochemical investigation on the stems of Garcinia bracteata collected from Xishuangbanna resulted in the isolation of a new flavone. By analysis of the HRESIMS, IR, UV, 1D and 2D NMR spectra, the structure of the new compound was determined as 7-methoxy-4',6-dihydroxy-8-isobutyryl-flavone(1). Compound 1 was also tested for its anti-tobacco mosaic virus(TMV) activity. Results suggested the 1 possessed remarkable anti-TMV activity, with an inhibition rate of 28.2%.